Data From:
Loregian A, Appleton BA, Hogle JM, Coen DM. 2004. "Specific residues in the connector loop of the human cytomegalovirus DNA polymerase accessory protein, UL44, are crucial for interaction with the UL54 catalytic subunit." J. Virol. 78: 9084-92. (Pubmed)
Supplementary material
List of primer pairs used for creation of UL44 mutants.
| Mutant | Forward primer | Reverse primer |
|---|---|---|
| C129A | 5’-agttcagctcggccgccgtgc acggccaagac-3’ |
5’-gtcttggccgtgcacggcggc cgagctgaactc–3’ |
| V130A | 5’-ctcggcctgcgcgcacggcca agacattg-3’ |
5’–caatgtcttggccgtgcgcgc aggccgagc–3’ |
| H131A | 5’-gctcggcctgcgtggccggcc aagacattgtg-3’ |
5’–gcacaatgtcttggccggcc acgcaggccgagc–3’ |
| G132A | 5’-gcctgcgtgcacgcccaagac attgtgcgc-3’ |
5’–gcgcacaatgtcttgggcgt gcacgcaggc–3’ |
| Q133A | 5’-gcgtgcacggcgcagacattg tgcgcg-3’ |
5’–cgcgcacaatgtctgcgccgt gcacgc–3’ |
| D134A | 5’-gtgcacggccaagccattgtg cgcgaaagc-3’ |
5’–gctttcgcgcacaatggcttg gccgtgcac–3’ |
| I135A | 5’-gtgcacggccaagacgctgtg cgcgaaagcg-3’ |
5’–cgctttcgcgcacagcgtctt ggccgtgcac–3’ |
| V136A | 5’-cacggccaagacattgcgcgc gaaagcgag-3’ |
5’–ctcgctttcgcgcgcaatgtc ttggccgtg–3’ |
| R137A | 5’-ggccaagacattgtggccgaa agcgagaattcggc-3’ |
5’–gccgaattctcgctttcggcc acaatgtcttggcc–3’ |
| E138A | 5’-gacattgtgcgcgcaagcgag aattcggcc-3’ |
5’–ggccgaattctcgcttgcgcg cacaatgtc–3’ |
| S139A | 5’-gacattgtgcgcgaagccgag aattcggccgtg-3’ |
5’–cacggccgaattctcggcttc gcgcacaatgtc–3’ |
| E140A | 5’-gtgcgcgaaagcgcgaattcg gccgtgcac-3’ |
5’–gtgcacggccgaattcgcgc tttcgcgcac–3’ |
List of primer pairs used for creation of UL44 truncated proteins.
| Construct | PCR round | Forward primer | Reverse primer |
|---|---|---|---|
| GST-UL44DC260 | First round | 5’-CTGGAAGTTCTGTTCC AGGGGCCCCTCGAGATG GATCGCAAGACGCG-3’ |
5’-AGCTGGGTCACGCGTC TATCCGTAACTATACGCAAC-3’ |
| Second round | 5’-GGGGACAAGTTTGTA CAAAAAAGCAGGCTTACTGGAAGTTCTGTTCCAG-3’ |
5’-GGGGACCACTTTGTAC AAGAAAGCTGGGTCACGCGTCTA-3’ |
|
| GST-UL44DC315 | First round | 5’-CTGGAAGTTCTGTTCC AGGGGCCCCTCGAGATGGATCGCAAGACGCG-3’ |
5’-AGCTGGGTCACGCGTC TACAGACCCGGGCCGTCGTC-3’ |
| Second round | 5’-GGGGACAAGTTTGTA CAAAAAAGCAGGCTTACTGGAAGTTCTGTTCCAG-3’ |
5’-GGGGACCACTTTGTAC AAGAAAGCTGGGTCACGCGTCTA-3’ |
|
| GST-UL44DC340 | First round | 5’-CTGGAAGTTCTGTTCC AGGGGCCCCTCGAGATGGATCGCAAGACGCG-3’ |
5’-AGCTGGGTCACGCGTC TATCCGTAACTATACGCAAC-3’ |
| Second round | 5’-GGGGACAAGTTTGTAC AAAAAAGCAGGCTTACTGGAAGTTCTGTTCCAG-3’ |
5’-GGGGACCACTTTGTAC AAGAAAGCTGGGTCACGCGTCTA-3’ |